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KMID : 0613820080180091257
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Journal of Life Science
2008 Volume.18 No. 9 p.1257 ~ p.1262
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The Anticancer Effect of Combination of Genistein and Photofrin PDT in Human AMC-HN3 Head and Neck Cancer Cell Lines
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Kang Jung-Wook
Ahn Jin-Chul
Chung Phil-Sang
Shin Jang-In
Son Seung-Yeol
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Abstract
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Photodynamic therapy (PDT) is a treatment utilizing the generation of singlet oxygen and other reactive oxygen species (ROS), which selectively accumulated in target cells. Genistein, soy-derived phytoestrogen, is one of the anticancer agents found in soybean. In the current study, we investigated the effect of photofrin-induced PDT and genistein on apoptotic cell death in head and neck cell line (AMC-HN3) to confirm the photodynamic therapy of genistein. It was determined by MTT assay that the combination group had more cytotoxicity effect than PDT group alone. Combination of photofrin PDT and genistein induced apoptosis more when comparing with PDT alone. Our data also showed that ROS was increased in combination therapy, indicating apoptosis by mitochondrial damage. These results indicated that the combination of photofrin PDT and genistein showed more cytotoxic effect and induced apoptosis in head and neck cancer cell line.
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KEYWORD
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Photodynamic therapy (PDT), genistein, photofrin, cytotoxicity effect, reactive oxygen species (ROS)
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